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This study has aimed to investigate the imutant influence of three insect growth regulators (IGRs): Neporex and Apploud, which belong to chitin synthesis inhibitors (CSIs), and Admiral, which belong to juvenile hormone analog JHAs. In Iraq , Kerbala Governorate of December 2022 . These IGRs are used to insect control by using biological methods including the study of reversal mutation for two strains of Salmonella typhimurium (TA100, TA98) in two ways: The plate incorporation method and the fluctuation method. The results showed that the CSIs (Neporax, Applaud) have mutant influence in bacteria cells for all used concentrations( which are prepared after determining the minimal inhibition concentration for each growth regulator). The plate incorporation method, It has been noticed that the numbers of reversal colonies in each strain increased with increasing concentrations compared with spontaneous reversal colonies in negative control. In using the fluctuation methods, It has been noticed increasing the turbid hole numbers which shows the material effect on bacteria mutation. The result referred to the positive relationship between concentration and the number of holes when testing Admiral (JHIs) by plate incorporation and fluctuation methods, the result has shown there is no mutant effect on Salmonella typhimurium. The statistical analysis has shown there are no significant differences between the numbers of reversal colonies and unclear holes as a result of different concentration treatments of JHIs with negative control of both strains (TA100, TA98)under testing.


Ames Carcinogenic Insect growth regulators Mutation

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Alaa Sajjad AlKhafagi, & Sarah Kadhim Al-Rahimy. (2024). Bacterial Reverse Mutation Test for Two Salmonella Typhimurium (TA100, TA98) Strains to Detect Mutant and Carcinogenic Effect of Some Insect Growth Regulators. Central Asian Journal of Theoretical and Applied Science, 5(1), 104-110. Retrieved from


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