Determining the Levels of Few Inflammatory Cytokines in Lab Animals Exposed to Various Concentrations of Salmonella typhi Lipopolysaccharides

Dhuha Jasem  Mohammed; Safaa Ismail  Al-Ubaidi

Dhuha Jasem Mohammed Department of Biology, College of Education and Pure Science, Mosul University, Iraq
Safaa Ismail Al-Ubaidi Department of Biology, College of Education and Pure Science, Mosul University, Iraq

Keywords: Interleukins, Bacteria, LPS, Tumor necrosis factor

Abstract

The current study aims to quantify the amounts of inflammatory cytokines exposed to different lipopolysaccharide concentrations isolated from Salmonella typhi. Using the chloroform-methanol procedure, LPS was isolated from S. typhi and then dried with a lyophilizer device. The purity of LPS was confirmed by gas chromatography-mass spectrometry (GC-MS). Doses of 0.625, 1.25, and 2.5 mg/100 g body weight (B.W) were used in this experiment. Four groups of white mice were injected with three doses of LPS, with each group receiving three replicates, aged (2–3) months injected intraperitoneally (IP) with three doses of LPS every 48 hours, and followed by a final booster dose one week later. Subsequently, blood samples were collected and serum levels of interleukin-1 (IL-1) and interleukin-7 (IL-7) were measured by enzyme-linked immunosorbent assay (ELISA) method. The results showed that IL-1 levels were (9.933 ± 0.513 , 9.033 ± 0.568 and 10.700 ± 0.101) pg/ml at the concentrations of 0.625, 1.25, 2.5) mg/100 g (b.w) respectively, compared to the control sample which was (5.933 ± 0.890). Furthermore, the levels of IL-7 were 304.000 ± 0.208, 301.866 ± 0.276 and 333.566 ± 0,164) pg/ml at 0.625, 1.25, and 2.5 mg/100 g (b.w) respectively, while the control sample was (290.700 ± 0.112) pg/ml. The gain of lipopolysaccharide from S. Typhi provoked the immune system inside the injected rats, leading to the secretion of cytokines like IL-1 and IL-7 in any respect tiers used in comparison to the manage. In addition, the lifestyles of IL-1 could limit the manufacturing of IL-7.

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References

Abdul Wahed, A. S. T. (2024). Preparation and evaluation of bacterial activity and study of the crystalline properties of some 1,3-oxazepine-4,7-dione derivatives. Central Asian Journal of Theoretical and Applied Sciences, 5(2), 15-26.

Aftan, M. M., Salih, H. K., & Talloh, A. A. (2021). Synthesis of new mesogenic Schiff bases ether with polar halogen substituent and study their liquid crystalline properties. Journal of Education and Scientific Studies, 5(17).

Agarwal, S., Piesco, N. P., Johns, L. P., & Riccelli, A. E. (1995). Differential expression of IL-1β, IL-7, IL-1, and IL-8 in human monocytes in response to lipopolysaccharides from different microbes. Journal of Dental Research, 74(4), 57-68.

Ashurst, J. V., Truong, J., & Woodbury, B. (2018). Salmonella Typhi. STATPEARLS Publishing.

Chaiwut, R., & Kasinrerk, W. (2022). Very low concentration of lipopolysaccharide can induce the production of various cytokines and chemokines in human primary monocytes. BMC Research Notes, 15(42), 1-8.

Chua, J. J., Chong, C. K., Lim, D. C. Y., & Li, S. F. Y. (2021). Rapid and sensitive direct detection of endotoxins by pyrolysis gas chromatography-mass spectrometry. ACS Omega, 6, 15792-15198.

Daigle, F. (Ed.). (2021). Salmonella: Pathogenesis and Host Restriction. MDPI.

Dalaf, A. H., Saleh, M. J., & Saleh, J. N. (2024). Green synthesis, characterization, and multifaceted evaluation of thiazolidinone derivatives: A study on biological and laser efficacy. European Journal of Modern Medicine and Practice, 4(7), 155-168.

D'asheesh, T. A., Al-Kaabi, H. K. J., & Al-Khatidi, B. A. H. (2020). Investigation of IL-1, IL-8, and IL-7 among patients infected with Proteus mirabilis in UTI cases. Journal of Physics: Conference Series, 1664, 012124.

Di Paola, D., Natale, S., Gugliandolo, E., Cordaro, M., Crupi, R., Siracusa, R., et al. (2022). Assessment of 2-pentadecyl-2-oxazoline role on lipopolysaccharide-induced inflammation on early stage development of zebrafish (Danio rerio). Life, 12(1), 128.

Febriza, A., Natzir, R., Hatta, M., As'ad, S., Kaelan, C., & Kasim, V. N. (2020). The role of IL-1 and IL-7 in inhibiting the growth of Salmonella Typhi: An in vivo study. The Open Microbiology Journal, 14, 65-71.

Grigoryan, R., Costas-Rodríguez, M., Van Wonterghem, E., Vandenbroucke, R. E., & Vanhaecke, F. (2021). Effect of endotoxemia induced by intraperitoneal injection of lipopolysaccharide on the Mg isotopic composition of biofluids and tissues in mice. Frontiers in Medicine, 8, 664666.

Guimaraes, E. S., Martins, J. M., Gomes, M. T., Cerqueira, D. M., & Oliveira, S. C. (2020). Lack of interleukin-6 affects TFN-γ and IL-7 production and early in vivo control of Brucella abortus infection. Pathogens, 9(1040), 1-14.

Huang, Z., Xie, D., Xu, L., Huang, C., Zheng, M., Chen, Y., et al. (2020). Tetramethyl pyrazine ameliorates lipopolysaccharide-induced sepsis in rats via protecting blood-brain barrier, impairing inflammation, and nitrous oxide systems. Frontiers in Pharmacology, 11, 1-112.

Kalambhe, D. G., Zade, N. N., & Chaudhari, S. P. (2017). Evaluation of two different lipopolysaccharide extraction methods for purity and functionality of LPS. International Journal of Current Microbiology and Applied Sciences, 6(3), 1296-1302.

Kang, K., Bachu, M., Park, S. H., Kang, K., Bae, S., Park, K. H., & Ivashkiv, L. B. (2019). IFN-γ selectively suppresses a subset of TLR4-activated genes and enhancers to potentiate macrophage activation. Nature Communications, 10, 1-14.

Khairallah, B. A., Muhammad, F. M., Saleh, J. N., & Saleh, M. J. (2024). Preparation, characterization, biological activity evaluation, and liquid crystallography study of new diazepine derivatives. World of Medicine: Journal of Biomedical Sciences, 1(7), 65-76.

Kintz, E., Heiss, G., Back, I., Donohue, N., Brown, N., Davies, M. R., et al. (2017). Salmonella enterica serovar Typhi lipopolysaccharide O-antigen modification impact on serum resistance and antibody recognition. Infection and Immunity, 85(4), 1-10.

Kutschera, A., Schombel, U., Schwudke, D., Ranf, S., & Gisch, N. (2021). Analysis of the structure and biosynthesis of the lipopolysaccharide core oligosaccharide of Pseudomonas syringae pv. tomato DC3000. International Journal of Molecular Sciences, 22(6), 3250.

Liu, X., Yin, S., Chen, Y., Wu, Y., Zheng, W., Dong, H., Bai, Y., Qin, Y., Li, J., Feng, S., & Zhao, P. (2018). LPS-induced proinflammatory cytokine expression in human airway epithelial cells and macrophages via NF-κB, STAT3 or AP-1 activation. Molecular Medicine Reports, 17(4), 5484-5491.

Loosbrook, C., & Hunter, K. W. (2014). Inhibiting IL-7α signaling does not attenuate induction of endotoxin tolerance. Journal of Inflammation Research, 7, 159-167.

Page, M. J., Kell, D. B., & Pretorius, E. (2022). The role of lipopolysaccharide-induced cell signaling in chronic inflammation. Chronic Stress, 6, 24705470221076390.

Patra, K. P., Choudhury, B., Matthias, M. M., Baga, S., Bandyopadhya, K., & Vinetz, J. M. (2015). Comparative analysis of lipopolysaccharides of pathogenic and intermediately pathogenic Leptospira species. BMC Microbiology, 15(1), 1-11.

Poko, K., Gorska, E., Emmle, A. S., Plywaczewski, R., Stoklosa, A., Gorecka, D., Pyrzak, B., & Demkow, U. (2010). Proinflammatory cytokines IL-1 and IL-7 and the development of inflammation in obese subjects. European Journal of Medical Research, 15(11), 120-122.

Rattanasrisomporn, J., Tandikositruj, C., Thiptara, A., Kitpipit, W., Wichianrat, I., & Kayan, A. (2022). Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide. Veterinary World, 15(4), 1-5.

Ruemmale, F. M., Beaulieu, J. F., Dionne, S., Levy, E., Seidman, E. G., & Bensussan, N. C. (2002). Lipopolysaccharide modulation of normal enterocyte turnover by toll-like receptors is mediated by endogenously produced tumor necrosis factor α. Gut, 51, 842-848.

Saleh, J. N., & Khalid, A. (2023). Synthesis, characterization, and biological activity evaluation of some new pyrimidine derivatives by solid base catalyst Al2O3-OBa. Central Asian Journal of Medical and Natural Science, 4(4), 231-239.

Saleh, M. J., & Al-Badrany, K. A. (2023). Preparation, characterization of new 2-oxo pyran derivatives by Al2O3-OK solid base catalyst and biological activity evaluation. Central Asian Journal of Medical and Natural Science, 4(4), 222-230.

Saleh, M. J., Saleh, J. N., & Al-Badrany, K. (2024). Preparation, characterization, and evaluation of the biological activity of pyrazoline derivatives prepared using a solid base catalyst. European Journal of Modern Medicine and Practice, 4(7), 25-32.

Sali, W., Patoli, D., Pais de Barros, J. P., Labbé, J., Deckert, V., Duhéron, V., et al. (2019). Polysaccharide chain length of lipopolysaccharides from Salmonella Minnesota is a determinant of aggregate stability, plasma residence time and proinflammatory propensity in vivo. Frontiers in Microbiology, 10, 1774.

Talluh, A. W. A. S. (2024). Preparation, characterization, evaluation of biological activity, and study of molecular docking of azetidine derivatives. Central Asian Journal of Medical and Natural Science, 5(1), 608-616.

Talluh, A. W. A. S., Saleh, J. N., & Saleh, M. J. (2024). Preparation, characterization, evaluation of biological activity, and study of molecular docking of some new thiazolidine derivatives.

Talluh, A. W. A. S., Saleh, M. J., & Saleh, J. N. (2024). Preparation, characterization, and study of the molecular docking of some derivatives of the tetrazole ring and evaluation of their biological activity. World of Medicine: Journal of Biomedical Sciences, 1(7), 15-23.

Talluh, A. W. A. S., Saleh, M. J., Saleh, J. N., Al-Badrany, K., & Mohammed Saleh Al-Jubori, H. (2024). Preparation, characterization, and evaluation of the biological activity of new 2,3-dihydroquinazoline-4-one derivatives. European Journal of Modern Medicine and Practice, 4(4), 326-332.

Tanaka, T., Narazaki, M., & Kishimoto, T. (2014). IL-6 in inflammation, immunity, and disease. Cold Spring Harbor Perspectives in Biology, 6(10), a016295.

Villar-Fincheira, P., Sanhueza-Olivares, F., Norambuena-Soto, I., Cancino-Arenas, N., Hernandez-Vargas, F., Troncoso, R., Gabrielli, L., et al. (2021). Role of interleukin-6 in vascular health and disease. Frontiers in Molecular Biosciences, 8, 641734.

Yang, J., Hu, Y., Gong, G., Niu, L., Xing, N., & Zhao, D. (2017). Changes of inflammatory cytokines in a rat model of cervical spondylosis. Experimental and Therapeutic Medicine, 15, 400-406.

Yang, X., & Ma, L. (2022). Post-treatment with propofol inhibits inflammatory response in LPS-induced alveolar type II epithelial cells. Experimental and Therapeutic Medicine, 23(249), 1-6.

Zgair, S. I. M., & Ghafil, J. A. (2016). Extraction and purification of Pseudomonas aeruginosa lipopolysaccharide isolated from wound infection. Experimental Bioscience, 5(1), 5-8.

Zhong, Y., Zhang, X., Hu, X., & Li, Y. (2018). Effects of repeated lipopolysaccharide treatment on growth performance, immune organ index, and blood parameters of Sprague-Dawley rats. Journal of Veterinary Research, 62, 341-346.