The Combination  Effect of Silver Nanoparticle and Some Antibiotics Against Staphylococcus Aureus and E.Coli Isolated From Wounds and Burns in Kirkuk City

Iman Muhammed  Salih; Salah Salman Zain  Alabden; Hadi Alwan  Mohammed

Iman Muhammed Salih Department of Biology, Collage of Education for Pure Sciences, University of Kirkuk, Kirkuk, Iraq
Salah Salman Zain Alabden Department of Biology, Collage of Education for Pure Sciences, University of Kirkuk, Kirkuk, Iraq
Hadi Alwan Mohammed Department of Biology, Collage of Education for Pure Sciences, University of Kirkuk, Kirkuk, Iraq

Keywords: Staphylococcus Aurous, Escherichia Coli, Silver Nanoparticles, Wounds, Burins

Abstract

The study included the isolation and identificstion of some species of pathogenic bacteria from wounds and in burn patients in Kirkuk city during the period from January 2023 to March 2024. 100 samples were collected from in Azadi Teaching Hospital in Kirkuk City The isolates were diagnosed by routine methods, and the diagnosis was confirmed by using the Vitik Compact system. The percentage of wound samples was 55% and the percentage of burn samples was 45%. The results of the culture showed that 15 isolates of Staphylococcus aureus appeared at a rate of 23% and 13 isolates of Escherichia coli at a rate of 20%. The highest bacterial infection rate was in the age groups (60-75) years with 25 isolates 25% and the age group (1-30) years recorded the least infection with 17 17.5%, while the infection rate of males was higher than females at 37% and 27% for females. The effectiveness of the manufactured nanoparticles was tested against bacterial isolates, as five different concentrations were used: 3, 6, 12, 25 and 50 micrograms per ml. All concentrations showed inhibiting the growth of most bacterial isolates. The results showed that silver nanoparticles recorded the highest inhibitory activity at a concentration of 50 micrograms on E.coli and Staph. Aureus with inhibition diameters (19 and 13) mm, respectively. The results Showed the synergism between the particles and ampicillin against E.coli and S.aureus bacteria with an inhibition diameter of 30 and 36 mm, respectively.

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References

G. McKnight, J. Shah, and R. Hargest, "Physiology of the skin," Surgery (Oxford), vol. 40, no. 1, pp. 8–12, 2022.

M. A. Richard, C. Paul, T. Nijsten, P. Gisondi, C. Salavastru, C. Taieb, and EADV Burden of Skin Diseases Project Team, "Prevalence of most common skin diseases in Europe: a population-based study," J. Eur. Acad. Dermatol. Venereol., vol. 36, no. 7, pp. 1088–1096, 2022.

G. Mancuso, A. Midiri, E. Gerace, and C. Biondo, "Bacterial antibiotic resistance: the most critical pathogens," Pathogens, vol. 10, no. 10, p. 1310, 2021.

H. A. Mohammed and L. A. M. Al Shalah, "Applications of silver nanoparticles as antibiotics for pathogenic organisms: Bacteria, fungi, viruses, and algae," J. Univ. Babylon Pure Appl. Sci., vol. 28, no. 3, 2020.

H. Korbekandi and S. Iravani, "Silver nanoparticles, the delivery of nanoparticles," in Hashim Abbass A., Ed., InTech, 2012, ISBN: 978-953-51-0615-9.

A. W. Bauer, W. M. M. Kirby, J. C. Sherris, and M. Turck, "Antibiotic susceptibility testing by a standardized single disk method," Am. J. Clin. Pathol., vol. 45, no. 4_ts, pp. 493–496, 1966.

CLSI, "Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing," 33rd Informational Supplement, vol. 32, no. 3, Wayne, Pennsylvania, USA, 2024.

T. C. Jackson, T. O. Uwah, N. L. Ifekpolugo, and N. A. Emmanuel, "Comparison of antimicrobial activities of silver nanoparticles biosynthesized from some citrus species," Am. J. Nano Res. Appl., vol. 6, no. 2, pp. 54–59, 2018.

J. Shang and X. Gao, "Nanoparticle counting: towards accurate determination of the molar concentration," Chem. Soc. Rev., vol. 43, no. 21, pp. 7267–7278, 2014.

O. O. Adeniji, M. O. Ojemaye, and A. I. Okoh, "Antibacterial activity of metallic nanoparticles against multidrug-resistant pathogens isolated from environmental samples: nanoparticles/antibiotic combination therapy and cytotoxicity study," ACS Appl. Bio Mater., vol. 5, no. 10, pp. 4814–4826, 2022.

A. A. Majbas, A. F. Naser, H. A. Rady, A. R. Zaghir, and N. M. Habib, "Isolation and identification of bacterial burn wound infection and their antimicrobial resistance," Curr. Clin. Med. Educ., vol. 2, no. 8, pp. 244–253, 2024.

T. Faïs, A. Cougnoux, G. Dalmasso, F. Laurent, J. Delmas, and R. Bonnet, "Antibiotic activity of Escherichia coli against multiresistant Staphylococcus aureus," Antimicrob. Agents Chemother., vol. 60, no. 11, pp. 6986–6988, 2016.

A. A. Hussain, L. B. Kalil, and S. Z. Salah, "The effect of silver nanoparticles prepared using Aspergillus niger in some pathogenic bacteria," Kirkuk Univ. J. Sci. Stud. (KUJSS), vol. 12, no. 1, 2017.

H. A. Abbood and S. S. Zainalabden, "Effect of iron oxide (Fe₂O₃) nanoparticles synthesized by Teucrium polium L. on Cryptococcus neoformans isolated from environmental sources in Kirkuk city, Iraq," Cerrado: Agric. Biol. Res., Univ. Kirkuk, 2024.

J. P. McEvoy, K. Genc, P. Loi, and W. J. Walker, "Antimicrobial applications of silver nanoparticles to E. coli colony biofilms," Methods Mol. Biol. (Clifton, N.J.), vol. 2118, pp. 21–28, 2020.

R. M. H. and Z. D. D., "The antibacterial activity of silver nanoparticles and detection of the fnbA gene in S. aureus isolated from burn patients," Thi-Qar Med. J. (TQMJ), vol. 27, no. 1, 2024.

M. Seong and D. G. Lee, "Silver nanoparticles against Salmonella enterica serotype Typhimurium: role of inner membrane dysfunction," Curr. Microbiol., vol. 74, no. 11, pp. 661–670, 2017.

B. Khalandi et al., "A review on the potential role of silver nanoparticles and possible mechanisms of their actions on bacteria," Drug Res., vol. 67, no. 11, pp. 70–76, 2017.

A. Rogowska, K. Rafińska, P. Pomastowski, J. Walczak, V. Railean-Plugaru, M. Buszewska-Forajta, and B. Buszewski, "Silver nanoparticles functionalized with ampicillin," Electrophoresis, vol. 38, no. 21, pp. 2757–2764, Nov. 2017.

A. M. Alotaibi et al., "Silver nanoparticle-based combinations with antimicrobial agents against antimicrobial-resistant clinical isolates," Antibiotics (Basel), vol. 11, no. 9, p. 1219, Sep. 2022.

E. M. Halawani, A. M. Hassan, and S. M. F. Gad El-Rab, "Nanoformulation of biogenic cefotaxime-conjugated silver nanoparticles for enhanced antibacterial efficacy against multidrug-resistant bacteria and anticancer studies," Int. J. Nanomedicine, vol. 15, pp. 1889–1901, 2020.

A. Thirumurugan, P. Aswitha, C. Kiruthika, S. Nagarajan, and A. N. Christy, "Green synthesis of platinum nanoparticles using Azadirachta indica – An eco-friendly approach," Mater. Lett., vol. 170, pp. 175–178, 2016.

E. A. M. Hussein, A. A. Mohammad, F. A. Harraz, and M. F. Ahsan, "Biologically synthesized silver nanoparticles for enhancing tetracycline activity against Staphylococcus aureus and Klebsiella pneumoniae," Braz. Arch. Biol. Technol., vol. 62, 2019.