Response of rice genotypes (Oryza sativa L.) to foliar spray with Biozyme TF regulator
Abstract
In order to study the influence of the growth regulator Biozyme TF (foliar applications at three levels: 0, 1.5, and 3 mg L-1, at the physiological stage V3/V4, and then after 15 days) on some growth traits, yield and its components of five rice genotypes ((Anber 6, Anber 33, Pokkali, genotype 13, genotype 14) and some chemical properties, a field experiment was conducted with a RCBD with three replicates in a farmer's farm in Najaf Governorate during the 2024 agricultural season. The results showed that spraying with Biozyme growth regulator led to a clear increase in all studied vegetative growth traits in all genotypes, as the B2 spray treatment outperformed and recorded the highest average of leaf area, panicle length and dry weight, which amounted to 65.00 cm2, 17.832 and 79.00 respectively, compared to the control treatment B0, which recorded the lowest averages. Treatment B2 gave the highest average leaf content of Mg, Cu, Fe and Zn, reaching 243.451, 5.8378, 263.852 and 10.4206, respectively, while the control treatment T0 gave the lowest average for the mentioned traits. Genotype Anber 33 also outperformed other genotypes in the leaf content of copper (6.3911), zinc (10.6456), and iron 326.470.
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References
2. Gérardeaux E, Falconnier G, Gozé E, Defrance D, Kouakou P, Loison R, et al. Adapting rainfed rice to climate change: a case study in Senegal. Agron. Sustain. Dev. 2021; 41 (4): 1–16.
3. Fu J, Jian Y, Wang X, Li L, Ciais P, Zscheischler J. Extreme rainfall reduces one-twelfth of China’s rice yield over the last two decades. Nat Food 2023; 4: 416–426.
4. Al-Khafaji Z, Al-Burki, F. Study of the effect of salt stress and kinetin and their interaction on the growth and yield of wheat (Triticum aestivum L.). In IOP Conference Series: Earth and Environmental Science. 2021; 923(1): 012084.
5. Ahmad P, Ahanger M, Singh V, Tripathi D, Alam P, Alyemeni, M. (Eds.). Plant metabolites and regulation under environmental stress. Academic Press. 2018.
6. [6] Pantoja-Benavides, Daniel A, Garces-Varon G, Restrepo-Díaz H. "Foliar growth regulator sprays induced tolerance to combined heat stress by enhancing physiological and biochemical responses in rice." Frontiers in Plant Science. 2021;12: 702892.
7. Karanja B, Isutsa D, Aguyoh J. Climate change adaptation of potato (Solanum tuberosum L.): Influence of Biozyme ® rate on potato yield, quality and mineral nutrient uptake. 2013.
8. Hassan H, Mostafa E, Selah M.Effect of foliar spray with Biozyme and sitofex on yield and fruit characteristics of Grand Nain banana. Green Farming. 2009; 2(10):661–663.
9. Kumar P, Dube S, Chauhan V. Photosythentic response of bell pepper to Biozyme in relation to fruit yield. Vegetable Science.2000; 27:54–56.
10. Pramanik M, Dutta D, Samui I. Effect of Seaweeds on Growth and Yield of Boro Rice (Oryza sativa L.). Curr. J. Appl. Sci. Technol, 2020; 28-34.
11. Hussain M, Omer O, Mohammed H. Response yield and some growth parameters of bread wheat to nano and nitrogen fertilizers. Journal of University of Duhok. 2021; 24(1) : 73-81.
12. Shakir H. Effect of spraying with Biozym TF growth regulator on the growth and yield of three Cucurbita moschata genotypes. Euphrates Journal of Agricultural Science. 2022; 14(2).
13. Al-Ethari Y, Salman, M. N., Al-Burki, F. R. The Effect of the Growth Regulator Biozyme on some Antioxidants, Chemical Traits, Growth and Yield of Bread Wheat Genotypes. In IOP Conference Series: Earth and Environmental Science. 2025; 1449( 1): 012061.
14. Ramesh S, Sudhakar P., Elankavi S, Suseendran, K, Jawahar S. Effect of gibberellic acid (GA3) on growth and yield of rice (Oryza sativa L.). Plant Arch, 2019; 19(1): 1369-1372.
15. Zahir Z, Asghar H, Arshad M. Cytokinin and its precursors for improving growth and yield of rice." Soil Biology and Biochemistry. 2001; 33.3:405-408.
16. Abu Dahi Y, Al-Younis M. Plant nutrition guide. Baghdad: University of Baghdad; Ministry of Higher Education and Scientific Research, Iraq. 1988.
17. Walsh L. Instrumental Methods for Analysis of Soils and Plant Tissue. Soil Science Society of America, Inc. 1971; 185-208.
18. Allen R, Haskin M, Anderson K, Müller O. Neutron activation analysis for 39 elements in small or precious geologic samples. J. Radioanal. Chem. 1970; 6: 115–137.
19. Alrrawi K, Khalaf Allah M. Design and analysis of experiments agricultural.Faculty of Agriculture. Mosul University. Iraq.1980.
20. Sosnowski J, Truba, M, Vasileva V. The Impact of Auxin and Cytokinin on the Growth and Development of Selected Crops. Agriculture. 2023; 13(3): 724.
21. Durand N, Briand X, Meyer C. The effect of marine bioactive substances (NPRO) and exogenous cytokinins on nitrate reductase activity in Arabidopsis thaliana. Physiol. Plant.2003; 119:489–493.
22. Mitra B, Mandal B. Influence of Biozyme on growth and yield performance of rice in rice-rice cropping sequence. ORYZA-An International Journal on Rice.2012; 49(3):189–194.
23. Stirk W, Novák O, Strnad M, Van Staden J. Cytokinins in macroalgae. Plant growth regulation.2003; 41(1):13-24.
24. Armin M, Akbari S, Mashhadi S. Effect of time and concentration of Nano Fe foliar application on yield and yield components of wheat. International Journal of Bioscience.2014; 4(9):69-75.
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