Determining the Prevalence of Staphylococcus aureus Bacteria in Nasal Swabs from Restaurant Workers in Al- Kut City

Mohammed Hassan  Aubed; Anmar Wetheer  Almeahy; Malik  Rheem  Jabbar; Nagham Ali  Shahib; Hadi Hussein  Maktouf; Buzugh  Hamid

doi:10.51699/cajotas.v6i4.1596

Mohammed Hassan Aubed Public Health Laboratory, Bacteriology Unit, Al-Kut , MOH, Iraq
Anmar Wetheer Almeahy Public Health Laboratory, Bacteriology Unit, Al-Kut , MOH, Iraq
Malik Rheem Jabbar Public Health Laboratory, Bacteriology Unit, Al-Kut , MOH, Iraq
Nagham Ali Shahib Public Health Laboratory, Bacteriology Unit, Al-Kut , MOH, Iraq
Hadi Hussein Maktouf Public Health Laboratory, Bacteriology Unit, Al-Kut , MOH, Iraq
Buzugh Hamid Public Health Laboratory, Bacteriology Unit, Al-Kut , MOH, Iraq

DOI: https://doi.org/10.51699/cajotas.v6i4.1596

Keywords: Staphylococcus aureus, Clindamycin Inducible Resistance (D-test), MRSA, MSSA

Abstract

Antibiotic resistance represents a global challenge to modern medicine, and among the most critical resistant pathogens is Staphylococcus aureus, particularly methicillin-resistant strains (MRSA). This study aimed to determine the prevalence of Staphylococcus aureus and the patterns of antibiotic resistance among nasal swab samples collected from restaurant workers in Al-Kut City. A total of 110 nasal swabs were obtained from food handlers and analyzed using standard microbiological techniques, including selective culturing on mannitol salt agar, coagulase testing, and Vitek2 identification. The D-test was used to assess inducible clindamycin resistance in MRSA isolates. Of the samples analyzed, 23 tested positive for Staphylococcus aureus, with 16 identified as MRSA and 9 as methicillin-sensitive S. aureus (MSSA). Antibiotic susceptibility tests revealed varying resistance rates among isolates, including 60.8% resistance to cefoxitin, 73.9% to oxacillin, 43.4% to vancomycin, 65.2% to erythromycin, 43.4% to clindamycin, 47.8% to ciprofloxacin, 17.4% to gentamicin, and 4.4% to chloramphenicol. These findings suggest a high prevalence of S. aureus carriage and antibiotic resistance among food handlers, underscoring the necessity of implementing regular screening and treatment programs in the food service industry to mitigate the risk of staphylococcal foodborne infections and protect public health.

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References

A. Schmidt, S. Benard, and S. Cyr, “Hospital cost of staphylococcal infection after cardiothoracic or orthopedic operations in France: A retrospective database analysis,” Surg. Infect. (Larchmt)., vol. 16, no. 4, pp. 428–435, 2015.

M. Z. David and R. S. Daum, “Community-associated methicillin-resistant Staphylococcus aureus: Epidemiology and clinical consequences of an emerging epidemic,” Clin. Microbiol. Rev., vol. 23, no. 3, pp. 616–687, 2010.

G. F. Karim, S. S. AL-Salihi, Q. M. Atya, and K. S. Abass, “Aerobic and Anaerobic Bacteria in Tonsils of Different Ages with Recurrent Tonsillitis,” Indian J. Public Health Res. Dev., vol. 10, no. 9, pp. 1022–1026, 2019.

M. Emaneini et al., “Nasal carriage rate of methicillin resistant Staphylococcus aureus among Iranian healthcare workers: A systematic review and meta-analysis,” Rev. Soc. Bras. Med. Trop., vol. 50, no. 5, pp. 590–597, 2017.

A. Iyer et al., “High incidence rate of methicillin-resistant Staphylococcus aureus among healthcare workers in Saudi Arabia,” J. Infect. Dev. Ctries., vol. 8, no. 3, pp. 372–378, 2014.

G. Yilmaz et al., “Detection and prevalence of inducible clindamycin resistance in staphylococci,” J. Med. Microbiol., vol. 56, no. 3, pp. 342–345, 2007.

M. Kanerva et al., “Costs of an outbreak of meticillin-resistant Staphylococcus aureus,” J. Hosp. Infect., vol. 66, no. 1, pp. 22–28, 2007.

I. Ifeanyichukwu et al., “Community-acquired methicillin resistant Staphylococcus aureus (Ca–MRSA) carriage amongst tertiary School Students,” Am. J. Sci. Technol., vol. 2, no. 1, pp. 18–21, 2015.

F. D. Lowy, “Staphylococcus aureus infections,” N. Engl. J. Med., vol. 339, no. 8, pp. 520–532, 1998.

Q. Rao et al., “Staphylococcus aureus ST121: A globally disseminated hypervirulent clone,” J. Med. Microbiol., vol. 64, no. 12, pp. 1462–1473, 2015.

E. Chukwunonso et al., “Methicillin-Resistant Staphylococcus aureus: A Mini Review,” Health Sci., vol. 7, no. 1, pp. 122–127, 2018.

S. Y. C. Tong et al., “Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management,” Clin. Microbiol. Rev., vol. 28, no. 3, pp. 603–660, 2015.

T. Iwase et al., “Staphylococcus epidermidis Esp inhibits Staphylococcus aureus biofilm formation and nasal colonization,” Nature, vol. 465, 2010.

E. Y. Garoy et al., “Methicillin-resistant Staphylococcus aureus (MRSA): Prevalence and antimicrobial sensitivity pattern among patients—a multicenter study in Asmara, Eritrea,” Can. J. Infect. Dis. Med. Microbiol., 2019.

G. N. Forrest and D. W. Oldach, “Macrolides and clindamycin,” in Infectious Diseases, 3rd ed., S. L. Gorbach, J. G. Bartlett, and N. R. Blacklow, Eds. Philadelphia: Lippincott Williams and Wilkins, 2004, pp. 223.

R. Gadepalli et al., “Inducible clindamycin resistance in clinical isolates of Staphylococcus aureus,” Indian J. Med. Res., vol. 123, no. 4, pp. 571, 2006.

B. A. Forbes, D. F. Sahm, and A. S. Weissfeld, Bailey and Scott’s Diagnostic Microbiology, 12th ed. St. Louis: Mosby, 2007, pp. 166–167.

J. G. Collee et al., Mackie and McCartney Practical Medical Microbiology, 14th ed. Singapore: Longman, 1996.

P. M. Tille, Bailey and Scott’s Diagnostic Microbiology, 13th ed. St. Louis: Mosby Elsevier, 2014.

D. M. Baird, Practical Medical Microbiology, 14th ed. New Delhi: Churchill Livingston, 2006, pp. 245.

Clinical and Laboratory Standards Institute (CLSI), Performance Standards for Antimicrobial Susceptibility Testing, 29th ed., M100, Jan. 2019.

U. A. R. Hussein, “Evaluation of antibacterial activity of cinnamon and ginger extracts against vancomycin-resistant Staphylococcus aureus,” Thi-Qar Med. J., vol. 14, no. 2, pp. 75–93, 2017.

A. M. Alsamarai, H. M. Abbas, and Q. M. Atia, “Nasal carriage of methicillin-resistant Staph. aureus in food providers in Samarra City,” World J. Pharm. Pharm. Sci., vol. 4, pp. 50–58, 2015.

N. Hussein, R. S. Salih, and N. A. Rasheed, “Prevalence of MRSA in Hospitals and Community in Duhok,” Int. J. Infect., vol. 6, no. 2, 2019.

Ç. Sezer et al., “Food handlers: A bridge in the journey of enterotoxigenic MRSA in food,” J. Verbrauch. Lebensm., vol. 10, no. 2, pp. 123–129, 2015.

M. Nasrolahei et al., “Bacterial assessment of food handlers in Sari City, Iran,” J. Infect. Public Health, vol. 10, no. 2, pp. 171–176, 2017.

E. Ahmadi et al., “Prevalence of and risk factors for MRSA nasal carriage in the West of Iran,” BMC Infect. Dis., vol. 19, p. 899, 2019.

A. Ma’moun, F. Al-Shakhsheer, and M. M. Al-Ababneh, “Restaurant employees’ food handling practices in Irbid City, Jordan,” J. Tourism Hosp. Manag., vol. 5, no. 1, pp. 81–89, 2017.

K. B. Laupland et al., “Severe bloodstream infections: A population-based assessment,” PubMed, vol. 32, no. 4, pp. 992–997, 2004.

M. El-Shenawy et al., “Cross sectional study of skin carriage and enterotoxigenicity of Staphylococcus aureus among food handlers,” Open J. Med. Microbiol., vol. 4, pp. 16–22, 2014.

N. A. Alrazzak, “Synthesis and characterization of 1,3,4-oxadiazole derivatives,” IOP Conf. Ser. Mater. Sci. Eng., vol. 454, no. 1, p. 012096, 2018.

K. A. Davis et al., “MRSA nares colonization at hospital admission and its effect on subsequent MRSA infection,” Clin. Infect. Dis., vol. 39, no. 6, pp. 776–782, 2004.

K. L. Shobha, P. S. Rao, and J. Thomas, “Survey of Staphylococcus isolates and antibiogram with emphasis on methicillin resistance,” Indian J. Med. Microbiol., vol. 23, no. 3, pp. 186, 2005.

[34] M. A. Abdulrahman and A. I. Taher, “Prevalence of MRSA Among Food Handlers in Duhok City,” Sci. J. Univ. Zakho., vol. 6, no. 4, pp. 140–145, 2018.

J. S. Ferreira et al., “Food handler-associated MRSA in public hospitals in Salvador, Brazil,” Food Control, vol. 37, pp. 395–400, 2014.

T. Wolde, M. Abate, and L. Mehari, “Prevalence and Resistance Pattern of S. aureus among Food Handlers,” IJSRET, vol. 2, no. 1, pp. 476–478, 2016.

M. Radhakrishna et al., “Prevalence of MRSA carriage amongst health care workers in India,” J. Clin. Diagn. Res., vol. 7, no. 12, pp. 2697, 2013.

M. Askarian et al., “Nasal carriage of MRSA and its antibiotic susceptibility pattern in Iran,” Int. J. Infect. Dis., vol. 13, no. 5, pp. e241–e247, 2009.

K. Ali, B. Javad, and R. Mahdieh, “Prevalence of MRSA nasal carriage in West Iran,” BMC Infect. Dis., vol. 19, 2019.

M. S. Assafi et al., “Prevalence of S. aureus nasal colonization and its antibiotic sensitivity pattern,” Sci. J. Univ. Zakho., vol. 5, no. 1, pp. 7–10, 2017.

L. Vatansever et al., “Carriage rate and methicillin resistance of S. aureus in food handlers,” Springerplus, vol. 5, no. 1, p. 608, 2016.

M. O. Marwan et al., “Prevalence of MSSA and MRSA nasal carriage in food handlers in Lebanon,” Access Microbiol., vol. 1, 2019.

E. Askari et al., “High-level vancomycin-resistant S. aureus (VRSA) in Iran: A systematic review,” J. Med. Bacteriol., vol. 1, no. 3–4, pp. 53–61, 2012.

A. A. Alzolibani et al., “Documentation of VRSA among children with atopic dermatitis in Saudi Arabia,” Acta Dermatovenerol Alp Pannonica Adriat., vol. 21, no. 3, pp. 51–53, 2012.

G. F. Brooks et al., Jawetz, Melnick & Adelberg's Medical Microbiology, 24th ed. New York: McGraw-Hill, 2007, pp. 224–232.