Studies on Inbred Lines their Combining Ability and Development of High Productive Hybrid in Rice (Oryza sativa L.)

  • Dr. Vinod Bahadur Singh KVK, Sant Kabir Nagar, A.N.D. University of Agriculture & Technology, Ayodhya (U.P.)
  • Dr. Virendra Pratap Singh Sr. Scientist /Head, KVK, Sultanpur, A.N.D. University of Agriculture & Technology, Ayodhya (U.P.)
  • Dr. Manoj Kumar Singh SMS (Horticulture), KVK, Gonda, A.N.D. University of Agriculture & Technology, Ayodhya (U.P.)
  • Dr. S. S. Kashyap SMS (Anim. Sc.),KVK, Sant Kabir Nagar, A.N.D. University of Agriculture & Technology, Ayodhya (U.P.)
Keywords: inbred, high, productive, hybrid, inlines, rice, Oryza sativa L, grain, genotypes

Abstract

The technology of hybrid in rice (Oryza sativa L.)  utilizing heterosis is an essential requirement for achieving food security. The current study was aimed at assessing the genetic parameters and the gene actions of 15 yield-component traits associated with heterosis, in 9 new parental lines of hybrid rice and their generated hybrids. Five cytoplasmic male sterile (CMS) lines were crossed with four restorer (R) lines using twenty generated line × tester designation hybrid combinations. The results revealed that all the traits were controlled by additive and non-additive gene actions. However, the additive variance was the main component of the total genotypic variance. Assessment of the general combining ability (GCA) detected the best combiners among the genotypes. The hybrid combinations that expressed the highest-positive specific combining ability (SCA) for grain-yield were detected. The correlation between the GCA and SCA was evaluated. The hybrid crosses with high-positive heterosis, due to having a better parent for grain yield, were detected. The principal component analysis (PCA) recorded the first four principal axis displayed Eigenvalues >1 and existing variation cumulative of 83.92% in the genotypes for yield component characteristics. Three-dimensional plots corresponding to the studied traits illustrated that the genotypes Guang8A × Giza181, Quan-9311A × Giza179, II-32A × Giza181, and II-32A × Giza179 are classified as possessing superior grain yield.

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References

Verma RL, Katara JL, Sah RP, Sarkar S, Azaharudin TP, Patra BC, et al. CRMS 54A and CRMS 55A: Cytoplasmic male sterile lines with enhanced seed producibility. 2018, NRRI Newsletter. January-March 2018;39:1-14

Jonse JW. Hybrid vigour in rice. Journal of American Society of Agronomy. 1926;18:423-428

IIRR, Indian Institute of Rice Research, Technical Bulletin, 2019. IIRR: Hyderabad

Sampath S, Mohanty HK. Cytology of semi-sterile rice hybrid. Current Science. 1954;23:182-183

Chen S, Lin XH, Xu CG, Zhang Q. Improvement of bacterial blight resistance of “Minghui 63,” an elite restorer line of hybrid rice, by molecular marker-assisted selection. Crop Science. 2014;40:239-244

Tang H, Zheng X, Li C, Xie X, Chen Y, Chen L, et al. Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes. Cell Research. 2017;27:130-146

Wang ZH, Zou YJ, Li XY, Zhang QY, Chen LT, Wu H. Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell. 2006;18:676-687

Wang K, Gao F, Ji Y, Liu Y, Dan Z. ORFH79 impairs mitochondrial function via interaction with a subunit of electron transport chain complex III in Honglian cytoplasmic male sterile rice. New Phytologist. 2013;198:408-418

Zhang H, Xu C, He Y, Zong J, Yang X. Mutation in CSA creates a new photoperiod-sensitive genic male sterile line applicable for hybrid rice seed production. Proceeding National Academy of Science, USA. 2013;110:76-81

Rao GS, Deveshwar P, Sharma M, Kapoor S, Rao KV. Evolvement of transgenic male-sterility and fertility-restoration system in rice for production of hybrid varieties. Plant Molecular Biology. 2018;96:35-51

Zhou H, He M, Li1 Z, Chen L, Huang Z, Zheng S, et al. Development of commercial thermo-sensitive genic male sterile rice accelerates hybrid rice breeding using the CRISPR/Cas9-mediated TMS5 editing system. Scientific Reports. 2016;6:373-395

Hu J, Wang K, Huang W, Liu G, Gao Y. The rice pentatricopeptide repeat protein RF5 restores fertility in Honglian cytoplasmic male-sterile lines via a complex with the glycine rich protein GRP162. Plant Cell. 2012;24:109-122

Tao C, Ming L, Zya Z, Zhen Z, Ling Z, Qing Y, et al. Development and application of a functional marker associated with fertility restoring gene for BT-type cytoplasmic male sterility (CMS) in japonica rice. Chinese Journal of Rice Science. 2013;27(3):259-264

Itabashi E, Iwata N, Fujii S, Kazama T, Toriyama K. The fertility restorer gene, Rf2 for lead rice-type cytoplasmic male sterility of rice encodes a mitochondrial glycine-rich protein. The Plant Journal. 2011;65:359-367

Katara JL, Verma RL, Nayak D, Umakanta N, Ray S, Subudhi H, et al. Frequency and fertility restoration efficiency of Rf3 and Rf4 genes in Indian rice. Plant Breeding. 2017;136:74-82

Liu XQ, Xu EX, Li SQ, Hu J, Huang JY, Yang DC, et al. Inheritance and molecular mapping of two fertility-restoring loci for Honglian gametophytic cytoplasmic male sterility in rice (Oryza sativa L.). Molecular Genetics and Genomics. 2004;271:586-594

Fujii S, Toriyama K. Molecular mapping of the fertility restorer gene for ms-CW-type cytoplasmic male sterility of rice. Theoretical and Applied Genetics. 2005;111:696-701

Igarashi K, Kazama T, Toriyama K. A gene encoding pentatricopeptide repeat protein partially restores fertility in RT98-type cytoplasmic male-sterile rice. Plant Cell Physiology. 2016;57(10):2187-2193

Okazaki M, Kazama T, Murata H, Motomura K, Toriyama K. Whole mitochondrial genome sequencing and transcriptional analysis to uncover an RT102-type cytoplasmic male sterility-associated candidate gene derived from Oryza rufipogon. Plant Cell Physiology. 2013;54(9):1560-1568

Toriyama K, Kazama T. Development of cytoplasmic male sterile IR24 and IR64 using CW-CMS/Rf17 system. Rice. 2016;9:22-26

Published
2022-02-28
How to Cite
Dr. Vinod Bahadur Singh, Dr. Virendra Pratap Singh, Dr. Manoj Kumar Singh, & Dr. S. S. Kashyap. (2022). Studies on Inbred Lines their Combining Ability and Development of High Productive Hybrid in Rice (Oryza sativa L.). Central Asian Journal of Theoretical and Applied Science, 3(2), 148-157. Retrieved from https://cajotas.centralasianstudies.org/index.php/CAJOTAS/article/view/405